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父亲RLIM/Rnf12是生产牛奶的膜细胞的生存因素

Baowei Jiao¹, Hong Ma, Maxim N Shokhirev

¹Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA.

|May 1, 2012

概括

父亲Rnf12/RLIM对于雌性小鼠乳生产细胞的生存至关重要. 它的缺失阻止了乳腺分化和牛奶生产,突出显示了它在表观遗传中的作用.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
哺乳动物的繁殖方式
基因调节基因调节

背景情况:

在体细胞和胚外细胞之间,X染色体失活 (XCI) 是不同的.
在 trofhoblast 细胞中印制的 XCI 沉默了父亲的 X 染色体.
Rnf12 (ubiquitin结合酶Rnf12/RLIM) 对于印制XCI启动至关重要.

研究的目的:

研究Rnf12/RLIM在女性乳腺发育中的作用.
确定Rnf12淘汰对膜分化和牛奶产量的影响.
探索Rnf12在乳腺上皮细胞中的等位基贡献.

主要方法:

在雌性小鼠乳腺中,Rnf12的有条件淘汰.
在怀孕期间分析气膜分化和牛奶产量.
在乳腺上皮细胞中对Rnf12等位基因功能进行遗传分析.

主要成果:

绝杀Rnf12抑制乳腺膜分化和牛奶的产生.
缺少RLIM的乳腺膜细胞在分化过程中经历了亡.
由于非随机的母体XCI,父亲的Rnf12等位基因主要负责这些功能.
父亲的Rnf12/RLIM作为乳生产细胞的关键生存因素.

结论:

父亲的Rnf12/RLIM对于生产牛奶的膜细胞的生存至关重要.
乳腺上皮细胞中非随机的母体XCI涉及父亲的Rnf12等位基因.
研究结果表明,这对跨代表观遗传有影响.

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