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Updated: May 22, 2026

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
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Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

Published on: January 11, 2017

基于活动的探针用于胺激酶信号传递.

Kaelyn E Wilke1, Samson Francis, Erin E Carlson

  • 1Department of Chemistry, Indiana University, Bloomington, 47405, United States.

Journal of the American Chemical Society
|May 22, 2012
PubMed
概括

研究人员开发了一种基于活动的新型探测器,用于检测细菌的histidine kinase (HK) 活性. 这种探测器标记了活性HK蛋白,为研究双组分系统 (TCS) 和识别潜在药物点提供了新的策略.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...