克隆相关的视觉皮层神经元表现出类似的刺激特征选择性
1Division of Neurobiology, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.
Nature
|June 9, 2012
概括
同一个放射性克隆中的姐妹神经元在正在发育的视觉皮层中具有相似的定向偏好. 差距连接通信对于这种功能相似性至关重要,支持辐射单位假设.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
背景情况:
- 哺乳动物新皮层组织是柱状的,视觉皮层中的功能柱具有类似的方向偏好.
- 这些功能柱的发育基础,特别是由辐射质纤维形成的本体遗传柱的作用,仍然不清楚.
研究的目的:
- 调查发育中的哺乳动物视觉皮层中本体遗传列 (辐射克隆) 和功能列之间的关系.
- 为了确定相同的辐射克隆中的姐妹神经元是否表现出类似的方向偏好.
- 阐明细胞间通信的作用,特别是间隙结,在功能柱的成熟.
主要方法:
- 利用编码绿色光蛋白的逆转录病毒载体,标记小鼠视觉皮层中激发性神经元的辐射克隆.
- 在体内采用双光子成像来测量神经元反应特性和方向偏好.
- 使用Cx26 (Gjb2) 的主导阴性突变体的病毒表达和carbenoxolone的管理中断的间隙连接合.
主要成果:
- 同一个辐射克隆中的姐妹神经元表现出类似的方向偏好,而非姐妹神经元则没有.
- 间隙连接合的破坏显著降低了姐妹神经元之间的功能相似性.
- 这些发现与最近发现的姐妹神经元之间的偏好激发连接一致.
结论:
- 这项研究提供了直接证据支持辐射单位假设,连接本体遗传和功能列.
- 在早期发育过程中通过间隙连接进行细胞间通信,对于在视觉皮层中建立功能柱状组织至关重要.
- 这项研究统一了视觉皮层中本体遗传和功能柱的概念.
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