通过比较S. pombe和S. cerevisiae的遗传相互作用,揭示了功能性重定位
Adam Frost1, Marc G Elgort, Onn Brandman
1Department of Biochemistry, Huntsman Cancer Institute, University of Utah, School of Medicine, Salt Lake City, 84112, USA. frost@biochem.utah.edu
Cell
|June 12, 2012
概括
将酵母物种进行比较,发现了保存和重新定位的遗传相互作用. 关键的发现包括ER压力传感器的新作用,细胞分裂中的ESCRT因素,以及在线粒分裂中的STRIPAK复合体.
科学领域:
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 基因相互作用地图揭示了基因之间的功能关系.
- 通过比较物种间的遗传相互作用,可以突出发现保存和分离的生物过程.
研究的目的:
- 为了构建一个基因相互作用地图的schizosaccharomyces pombe (裂变酵母).
- 为了将这张地图与Saccharomyces cerevisiae (开花酵母) 的地图进行比较,以确定保存和分离的遗传关系.
- 为了研究正统基因复合体的功能性转换.
主要方法:
- 在S. pombe.中进行系统的双对基因相互作用选.
- 对S. pombe和S. cerevisiae之间的遗传相互作用图的比较分析.
- 实验验证S. pombe和哺乳动物细胞中发现的功能性重定位事件.
主要成果:
- 对于大约40%的非必不可少的S. pombe基因,生成了一个全面的遗传相互作用地图.
- 观察到广泛的遗传关系的保护,但也发现了 ortologous 综合体的显著功能性重用.
- 功能性重定位的三个具体实例得到了验证: (1) ER应激反应中的Ire1和Gpt1, (2) 螺旋杆体重复中的ESCRT因子,以及 (3) 线粒细胞进展中的STRIPAK复合体.
结论:
- 在裂变和芽酵母之间,遗传相互作用在很大程度上得到保护,但功能性重新定位推动了进化分歧.
- 鉴定到的功能性重定位事件揭示了在不同的细胞过程中保存的蛋白质复合体的新角色.
- 这些发现对理解遗传系统进化和在研究中利用模型生物有意义.
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