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克隆和表达一个发育调节的蛋白质,诱导神经元和神经元外生长活动

Y S Li¹, P G Milner, A K Chauhan

¹Department of Medicine, Jewish Hospital, Washington University Medical Center, St. Louis, MO 63110.

Science (New York, N.Y.)

|December 21, 1990

概括

一种新发现的蛋白质,类蛋白 (PTN),在物种中高度保存,并刺激细胞生长和神经元外生长. 其广泛的组织分布和发育调节表明它在生物过程中起着关键作用.

科学领域:

生物化学生物化学
发育生物学发展生物学
分子生物学分子生物学

背景情况:

从牛子宫中分离出一种与肝素结合的基蛋白.
它与新生小鼠大脑中的蛋白质有序相似性.

研究的目的:

隔离和描述编码这种蛋白质的牛,人类和老鼠基因的cDNA.
研究蛋白质的生物活动及其在发育中的潜在作用.

主要方法:

cDNA的分离和测序. cDNA的分离和测序.
蛋白质在COS-7细胞中的表达.
对NRK细胞的线粒激应活性进行检测.
在胚胎大鼠大脑细胞上进行神经元外生长测试.
在各种组织中进行RNA转录分析.

主要成果:

孤立的cDNA编码了高度保守的蛋白质,与已知的生长或神经营养因素无关,但与视网膜酸诱导的转录有50%的相同性.
该蛋白在NRK细胞上表现出代活性,并诱导神经元外生长.
RNA转录被广泛分布和发育调节.

结论:

这种蛋白质被更名为类蛋白 (PTN),表现出多样化的活动,可能是发育调节细胞因子家族的第一个成员.
PTN的自然保护和生物活动表明它在发展中发挥了重要作用.

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