相关实验视频
Updated: May 11, 2026

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Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
Published on: May 25, 2015
通过let-7和Argonaute对微RNA生物发生的自调节
Dimitrios G Zisoulis1, Zoya S Kai, Roger K Chang
1Division of Biology, University of California, San Diego, La Jolla, California 92093-0349, USA.
Nature
|June 23, 2012
概括
微RNAs (miRNAs) 通过准信使RNAs来调节基因表达. 这项研究表明,阿尔戈诺特蛋白还结合了let-7 miRNA初级转录,促进了它们的处理,并为miRNA生物发生创造了积极反循环.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 在RNA生物学,RNA生物学.
背景情况:
- 微RNAs (miRNAs) 是小型RNA分子,可以在转录后调节基因表达.
- miRNA生物生成涉及Drosha和Dicer处理的初级转录到成熟的miRNAs.
- 成熟的miRNA引导阿尔戈诺特蛋白向信使RNA,导致翻译抑制.
研究的目的:
- 为了调查miRNA路径是否针对非编码RNA.
- 阐明阿尔戈诺特蛋白在miRNA生物发生中的作用.
- 发现控制miRNA表达的新机制.
主要方法:
- 在Caenorhabditis elegans和人类细胞中研究了Argonaute蛋白与miRNA初级转录的结合.
- 分析了成熟的let-7miRNA及其主要转录之间的相互作用.
- 研究了阿尔戈诺特对let-7初级转录处理的影响.
主要成果:
- 阿尔戈诺特蛋白 (C. elegans中的ALG-1) 结合于let-7 miRNA初级转录.
- 成熟的let-7miRNA调解了这种相互作用,形成了一个正反循环.
- 阿尔戈纳特促进了跨物种的let-7 miRNA初级转录的生物发生.
结论:
- 该miRNA路径调节编码和非编码RNA.
- 阿尔戈纳特在促进miRNA生物发生方面发挥着新的作用.
- 莱特-7生物发生的自调节代表了控制miRNA表达的新机制.
相关概念视频
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

