DCAP:是一种广泛的抗生素,向细菌的细胞质膜
Ye-Jin Eun1, Marie H Foss, Daniela Kiekebusch
1Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Journal of the American Chemical Society
|June 30, 2012
概括
一种新的广谱抗生素,DCAP,通过破坏细胞膜,有效地向休眠和生物膜细菌. 这为耐传统抗生素的持续性感染提供了有前途的新疗法.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 药物发现 药物发现 药物发现
背景情况:
- 持久性感染通常涉及缓慢生长的,与生物膜相关的细菌.
- 需要快速细胞生长的常规抗生素对这些持久微生物无效.
- 膜向抗生素显示出对抗缓慢生长的细菌的潜力.
研究的目的:
- 为了引入一种新的膜活性化合物,DCAP.
- 评估DCAP对持久性和生物膜细菌的疗效.
- 评估DCAP在哺乳动物细胞上的安全性.
主要方法:
- 合成和表征的2 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
- 评估DCAP对细菌跨膜潜力和膜蛋白局部化 (MinD,FtsA) 的影响.
- 测试DCAP对缺乏营养的细菌和生物膜的抗菌活性,以及对红细胞和哺乳动物细胞的细胞毒性.
主要成果:
- 在DCAP的研究中,DCAP表现出广泛的抗微生物活性,对抗阳性和阴性细菌.
- DCAP降低了细菌的跨膜潜力,并导致了必要的膜蛋白的错位.
- DCAP有效地杀死缺乏营养的细菌,消毒生物膜,并表现出低的哺乳动物细胞毒性.
结论:
- 像DCAP这样的膜活性化合物是治疗持续性细菌感染的有希望的策略.
- DCAP代表了一种新的治疗性小分子,具有广泛的抗微生物应用潜力.
- DCAP为开发抗生素耐药性和持久性感染的治疗方法提供了新的途径.
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