概括
这项研究详细介绍了编码腺病毒2 (Ad2) DNA结合蛋白 (DBP) mRNA的领导者的DNA序列. 结果揭示了独特的5'末和缺乏典型的促进子序列,使其与其他病毒和细胞mRNA区别开来.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 遗传学 是一个遗传学.
背景情况:
- 腺病毒2 (Ad2) 感染涉及从多个早期阶段单元转录.
- 早期II区域为72KDNA结合蛋白 (DBP) 编码mRNA,这对DNA复制至关重要.
研究的目的:
- 阐明DBP mRNA的第一个领导者的结构.
- 为了分析这位领导者编码的侧面DNA序列.
- 将这些序列与已知的病毒和细胞mRNA结构进行比较.
主要方法:
- 在体内标记mRNA领导者与32P.
- 通过DNA过器杂交,将领导者分离到病毒限制片段Eco RI F.
- 对RNAase T1和RNAase A寡核酸的RNA指纹分析.
- 相关病毒片段的DNA测序.
主要成果:
- 在Hae III C亚片 (坐标75.1) 内的一个68核酸区域编码DBPmRNA领导者.
- 领导者的3'侧翼DNA包含一个拼接供体序列,与Ad2主要晚期领导者和小鼠球蛋白mRNA同源.
- 上游DNA缺乏其他转录单元中常见的"TA-TAAA"促进体同质性.
- DBP mRNA领导者表现出两个明显的5'封闭端子 (7meGpppA和7meGpppG),编码在相邻的DNA位置,导致5'末端.
结论:
- 它的DBPmRNA领导结构,包括5'端分离和缺乏上游同源性,使其与许多病毒和细胞mRNA区别开来.
- 这些特征使DBPmRNA与SV40和多瘤病毒的晚期信使保持一致.
- 这些发现提供了对控制mRNA 5'末端规范的机制的见解.
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