状树突细胞从无处不在的周围细胞前体中出现
Nike Julia Krautler1, Veronika Kana, Jan Kranich
1Institute of Neuropathology, University Hospital of Zurich, 8091 Zurich, Switzerland.
Cell
|July 10, 2012
概括
状树突细胞 (FDC) 源于表达血小板衍生生长因子受体β (PDGFRβ) 的周围血管前体. 这些前体可以在淋巴细胞器官之外分化为功能性FDC,解释了新的淋巴细胞组织的形成.
科学领域:
- 免疫学 免疫学 免疫学
- 发育生物学 发展生物学
- 细胞生物学 细胞生物学
背景情况:
- 毛囊树突细胞 (FDC) 对淋巴卵泡的结构和功能至关重要.
- FDC的起源和分化途径一直是免疫学中一个长期存在的问题.
- 了解FDC发育是理解淋巴组织组织和免疫反应的关键.
研究的目的:
- 为了确定负责FDC分化的前体细胞.
- 研究血小板衍生生长因子受体β (PDGFRβ) 在FDC发育中的作用.
- 探索周血管前体在已建立的淋巴体器官之外形成FDC的潜力.
主要方法:
- 利用PDGFRβ-Cre记者基因系统来追踪PDGFRβ表达细胞的血统.
- 进行PDGFRβ表达细胞的条件切除,以评估它们对于FDC形成的必要性.
- 在接受者小鼠中移植脂肪组织衍生的血管细胞和细胞,以研究体内FDC分化.
- 研究了淋巴毒素α和淋巴组织诱导细胞在FDC前体扩张中的作用.
主要成果:
- 证明FDC源于表达PDGFRβ.的无处不在的周围血管前体 (preFDC).
- 通过基因废除研究证实PDGFRβ(+) 细胞对FDC发育至关重要.
- 表明preFDC存在于淋巴体器官之外,并且可以在适当的信号发送时分化为功能FDC,例如在淋巴毒素α过度表达的脏中.
- 鉴定了脂肪组织衍生的PDGFRβ (((+) 流体血管细胞能够分化为成熟的FDC,具有免疫复合物捕获和B细胞招募能力.
- 揭示了周周血管FDC前体在淋巴细胞缺乏的脏中的扩张需要LTi细胞和淋巴毒素.
结论:
- 状树突细胞起源于表达PDGFRβ.的周围血管前体.
- 这些前体分布广泛,可以在淋巴细胞器官外分化为功能性FDC,从而导致de novo淋巴细胞组织的形成.
- 这些发现阐明了FDC的关键发育途径,并对了解免疫系统组织和炎症反应产生影响.
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