一个光照明的Ag纳米团探测器,通过发射颜色来区分单核酸变体
Hsin-Chih Yeh1, Jaswinder Sharma, Ie-Ming Shih
1Center of Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop K771, Los Alamos, New Mexico 87545, USA. tim.yeh@austin.utexas.edu
Journal of the American Chemical Society
|July 11, 2012
概括
研究人员开发了一种新的蝶纳米集群信标 (cNCB) 用于快速检测遗传变异. 这种简单的,无酶的光探测器可以准确地识别单核酸多态 (SNP) 与颜色的变化,使裸眼识别.
科学领域:
- 生物化学 生物化学
- 纳米技术 纳米技术
- 分子生物学分子生物学
背景情况:
- 选基因变异,如单核酸多态 (SNP) 对于诊断至关重要,但在护理场所设置具有挑战性.
- 快速基因分析需要无酶,易于使用的分辨探针.
- 纳米集群信标 (NCB) 是DNA模板的银色纳米集群,具有特定序列的光.
研究的目的:
- 开发一种新的光分子探针,用于直接检测SNP而不需要酶反应.
- 创建一个探针,在与不同的SNP目标结合时呈现出明显的颜色变化.
- 建立一个简单,低成本,快速的SNP识别方法,适合于点的护理应用.
主要方法:
- 基于银纳米集群和DNA增强器对齐的蝶纳米集群信标 (cNCBs) 的开发,可以改变光颜色.
- 利用SNP依赖的调整变异来区分遗传标.
- 对合成DNA,与疾病相关的SNP和临床患者样本的cNCB方法的验证.
主要成果:
- 在通过SNP特定对齐调节的cNCB中证明了显著的光发射颜色变化 (60-70nm).
- 在各种DNA标中成功检测了所有单核酸替代场景.
- 验证了该方法对与疾病相关的SNP和来自卵巢癌患者的临床样本的疗效.
结论:
- 驼纳米集群信标为直接SNP检测提供了一种新的,无酶的方法.
- 该cNCB方法提供了一个可靠的,低成本的测定与简单的视觉读取为基因变异查.
- 这项技术对推进护理点遗传诊断有前途.
相关概念视频
Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
FISH - Fluorescent In-situ Hybridization
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...


