聚合体沉声器的定量调制是本地化自然变化的基础
Vincent Coustham1, Peijin Li, Amy Strange
1John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
植物对气候的适应依赖于开花时间,由本地化调节. 对FLC基因的表观遗传沉默,涉及到基因组修饰 (H3K27me3),是关键. 在FLC的遗传变异影响这个过程,影响冷反应和进化.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 进化遗传学的进化遗传学
背景情况:
- 阿拉比多普西斯 (Arabidopsis thaliana) 呈现出多样化的气候适应,开花时间和本地化反应对生存至关重要.
- 寒冷诱导的开花过程Vernalization,涉及到由Polycomb Repressive Complex 2 (PRC2) 对FLOWERING LOCUS C (FLC) 基因的表观遗传沉默.
- 在 lysine 27 (H3K27me3) 中,PRC2 调解了组分离子 H3 三甲基化,从而导致 FLC 基因沉默.
研究的目的:
- 调查Arabidopsis thaliana冬季长度的本土化反应变化的遗传基础.
- 确定FLC基因内的cis作用多态如何影响表观遗传沉默和寒冷的要求.
主要方法:
- 定量特征位点 (QTL) 地图绘制以确定控制冬季长度反应的遗传位点.
- 精细映射和测序以精确确定FLC内的致病多态.
- 染色体免疫沉 (ChIP) 试验用于评估FLC中的H3K27me3水平,以应对遗传变异.
主要成果:
- 冬季长度的变化对FLC基因内的cis多态反应映射.
- 在FLC核化区域附近的四种多态的特定组合要求更长的冷暴露.
- 这些多态变异改变了FLC核化部位和基因体中的H3K27me3积累,影响了表观遗传沉默效率.
结论:
- 在FLC的Cis-regulatory变化是适应当地冬季条件的开花时间的一个重要因素.
- 通过cis变异对染色质沉默的定量调制提供了进化适应的机制.
- 了解这些表观遗传机制对于预测植物对环境变化的反应至关重要.
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