在一个代的多基酸合成模块中,甲基酸中间体的间逆转移
Benjamin Busch1, Nico Ueberschaar, Yuki Sugimoto
1Leibniz Institute for Natural Product Research and Infection Biology, HKI, Jena, Germany.
Journal of the American Chemical Society
|July 18, 2012
概括
研究人员调查了在 aureothin 生产中代多基酸合成酶模块的使用情况. 结果表明,多基基的中间转移从ACP回到相反链上的KS领域,而不是通过ACP-ACP航班.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 合成生物学 合成生物学
背景情况:
- 聚乙合成酶 (PKS) 对于生产各种自然产品至关重要.
- 在PKS模块利用的代机制仍然不完全理解.
- 欧列的生物合成涉及一个复杂的PKS组装线.
研究的目的:
- 为了阐明PKS模块在 aureothin组件中的代机制.
- 研究特定域的作用,包括AuraA的N端,在PKS代中的作用.
- 测试生物合成过程中聚基酸中介物转移的替代模型.
主要方法:
- 在黄素PKS组装线内的向域失活.
- 具有特定域删除的PKS异构体的构造和功能分析.
- 在PKS删除突变体 (ΔaurA) 中进行补充研究.
主要成果:
- 发现AuraA的N端对于代PKS过程是不可或缺的.
- 一个AurA(KS°,ACP°) -AurA(AT(0)) 异构体是非功能性的,这表明KS和ACP领域的关键作用.
- 在一个DaurA突变体中恢复金色素生产,并补充了AurA ((KS°) -AurA ((ACP°) 支持一个特定的转移模型.
结论:
- 这项研究驳斥了对代PKS函数的ACP-ACP穿模型.
- 有证据支持一种模型,在该模型中,与ACP结合的多基基介质被转移回 PKS 链对面的 KS 域.
- 这项工作阐明了像青素这样的多基基体复杂生物合成的关键步骤.
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