一个核阿尔戈诺特促进了多代表观遗传和生殖系不朽
Bethany A Buckley1, Kirk B Burkhart, Sam Guoping Gu
1Laboratory of Genetics, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Nature
|July 20, 2012
概括
在C. elegans中,多代表观遗传是由HRDE-1蛋白质介导的,该蛋白质通过几代人传输RNA干扰 (RNAi) 沉默信号,以维持生殖线功能并防止不育.
科学领域:
- 遗传学 是一个遗传学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 表观遗传信息通常会在几代人之间被删除.
- 多代表观遗传,即特征被传承下来,在一些生物体中发生.
- 在C. elegans中,双链RNA介导的基因沉默 (RNAi) 是这种遗传的一个已知的例子,持续超过五代.
研究的目的:
- 为了识别参与传输RNAi沉默信号的基因,在C. elegans.中跨越几代人.
- 阐明了多代表观遗传背后的分子机制.
主要方法:
- 进行了基因选,以发现线虫在遗传性RNAi中有缺陷.
- 鉴定并描述了遗传性RNAi缺陷1 (hrde-1) 基因.
- 研究了HRDE-1与小干扰RNA的关联及其在基因素修饰中的作用.
主要成果:
- 屏幕识别了hrde-1基因,编码一个阿尔戈诺特蛋白质,对RNAi遗传至关重要.
- HRDE-1 与生殖细胞中的小干扰RNA结合,并将组织素H3三甲基化 (H3K9me3) 引导到目标位置.
- HRDE-1或核RNAi的损失导致渐进的生殖线沉默,性质细胞功能障碍和不育.
结论:
- HRDE-1对于指导促使多代RNAi遗传的生殖基因沉默至关重要.
- 这种RNAi遗传机制促进了C. elegans的生殖细胞血统的不朽.
- C. elegans利用RNAi遗传来跨代传递表观遗传信息,以调节生物过程.
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