转录,拼接和释放:我们已经到了吗?
Ranjan Sen1, Sebastian D Fugmann
1Laboratory of Molecular Biology and Immunology, National Institute on Aging, 251 Bayview Boulevard, Baltimore, MD 21224, USA. ranjan.sen@nih.gov
Cell
|July 24, 2012
概括
根据Bhatt等的说法,巨细胞的炎症反应是由转录启动控制的. 具有内子的全长RNA积聚在染色质上进行处理,然后成为功能mRNA.
科学领域:
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
- 基因规则 基因规则
背景情况:
- 脂多糖 (LPS) 是巨细胞中炎症反应的强有力的激活剂.
- 基因表达调节对于理解细胞对LPS等刺激的反应至关重要.
- 以前的研究表明,转录后调节在炎症基因表达中起着重要作用.
研究的目的:
- 系统地分析LPS刺激后巨细胞的基因表达变化.
- 研究控制炎症基因表达的调节机制.
主要方法:
- 对基因表达的系统分析.
- 对新生RNA积累和处理的分析.
- 基于染色体的RNA研究.
主要成果:
- 炎症反应主要在转录启动的水平上受到调节.
- 含有内子的全长新生RNA积聚在染色体上.
- 在功能性mRNA释放和细胞质输出之前,内部RNA积累.
结论:
- 转录启动是LPS诱导的炎症基因表达的一个关键控制点.
- 新生的RNA处理和染色质保留在mRNA生产中起着重要作用.
- 这表明了一种与转录集成的新型的转录后调节层.
相关概念视频
01:10Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
01:10Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
01:17Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
01:17Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...