相关实验视频
Updated: May 11, 2026

14:06
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
Published on: April 16, 2013
突变在急性髓性白血病的起源和演变
John S Welch1, Timothy J Ley, Daniel C Link
1Department of Medicine, Washington University, St. Louis, MO 63110, USA.
Cell
|July 24, 2012
概括
大多数急性髓性白血病 (AML) 突变在发病事件发生之前,在造血干细胞/原生细胞中随机发生. 这些细胞捕获了它们的突变史,需要很少的额外突变来促进癌症的发展和进展.
科学领域:
- 基因组学就是基因组学.
- 癌症生物学 癌症生物学
- 血液形成 血液形成
背景情况:
- 基因组突变通常是在启动后获得的,推动了癌症的进化.
- 急性髓性白血病 (AML) 往往呈现出正常的肌型,缺乏基因组的不稳定性,挑战了这个模型.
- 了解AML中的突变获取对于破译克隆进化至关重要.
研究的目的:
- 为了调查急性髓性白血病 (AML) 突变的起源.
- 在AML基因组中区分发病前和发病后事件突变.
- 为了阐明AML的克隆进化过程,具有不同的细胞遗传特征.
主要方法:
- M3-AML (PML-RARA阳性) 和正常型M1-AML样本的全基因组测序.
- 来自健康个体的血造干细胞/原生细胞 (HSPC) 的外体序列测序.
- 对不同细胞类型和AML亚型的突变格局进行比较分析.
主要成果:
- 大多数AML基因组突变似乎是在引发突变之前在HSPC中随机获得的.
- 细胞的突变史是早期建立的,并在克隆扩张期间"捕获".
- 少数额外的合作突变 (通常是1-2) 足以形成恶性创始克隆.
结论:
- AML突变在很大程度上是先前存在的,HSPC中的随机事件,不仅仅是由启动后的基因组不稳定性驱动的.
- 克隆扩张"记录"创始细胞的突变历史.
- 在创始克隆中随后的合作突变驱动疾病的进展和复发.
相关概念视频
Hematopoiesis
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
Lineage Commitment
Commitment is the process whereby stem cells:
Differentiation of Common Myeloid Progenitor Cells
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
Regulation of Hematopoietic Stem Cells
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Overview of Hematopoiesis
Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
Bone Marrow Sampling and Transplants
Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy the...
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy the...

