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Phase Transitions and Effect of Intermolecular Forces
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在酵母菌中,细胞循环合的中心核核细胞的结构振荡
Manjunatha Shivaraju1, Jay R Unruh, Brian D Slaughter
1Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
Cell
|July 24, 2012
概括
发芽的酵母中间体在细胞周期的大部分时间里都含有基因素变体Cse4 (中心核细胞体) 的一个副本. 这种结构在亚纳相期间翻倍为两个副本,表明细胞循环合的细胞核细胞结构的变化.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 中心分子对于细胞分裂期间精确分离染色体至关重要.
- 中介质的特点是专门的核细胞体含有素H3变体.
- 在芽的酵母中,Cse4是中粒体中发现的正规的组素H3变体.
研究的目的:
- 为了研究活酵母细胞中中心基质素H3变体Cse4的复制数和结构动力学.
- 探索细胞循环依赖的细胞核细胞组合的变化.
主要方法:
- 开发了一种结合光相关谱 (FCS) 和校准成像的新方法,用于对活酵母进行定量分析.
- 使用核细胞复合和ChIP (染色体免疫沉) 试验来分析核细胞组合.
- 研究了Cse4-Cse4相互作用和Scm3在细胞周期中的作用.
主要成果:
- 定量Cse4拷贝数在中心核细胞核体中,在间相期间显示一个拷贝,在无相期间显示两个拷贝.
- 观察到细胞循环合的细胞核细胞的结构变化,与增加的Cse4合并和沉积在亚纳相.
- 在Cse4结构中证实了H2A/H2B的存在,并在Candida albicans中观察到类似的亚纳相特异性变化.
结论:
- 中心核体在其结构中表现出细胞周期合的振荡,特别是关于Cse4拷贝数的振荡.
- 这些发现支持了在细胞分裂过程中发生变化的中心核细胞体结构的动态模型.
- 观察到的现象在不同的酵母菌种中得到保存,这表明它具有根本性的重要性.
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