在细菌中构成洞穴的组成性形成
Piers J Walser1, Nicholas Ariotti, Mark Howes
1The University of Queensland, Institute for Molecular Bioscience, Brisbane, Queensland 4072, Australia. piers.walser@nhsbt.nhs.uk
Cell
|August 21, 2012
概括
细菌中的洞穴蛋白表达形成洞穴状囊泡. 这些异质洞穴模仿本地结构,并可以封装分子以提供有针对性的输送.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 结构生物学 结构生物学
背景情况:
- 洞穴蛋白是形成洞穴的必要蛋白质,动物细胞中的重要表面坑.
- 洞穴形成的精确机制仍然不完全理解.
研究的目的:
- 在简化 prokaryotic 系统中研究洞穴的自我组装和膜芽.
- 为了确定洞穴是否可以在哺乳动物细胞外形成功能性的洞穴状结构.
主要方法:
- 野生类型和突变洞穴蛋白在缺乏细胞内膜的 prokaryotic 主体中的表达.
- 低温电子断层扫描用于分析卡韦林诱导囊泡的结构和排列.
- 来自细胞质膜的囊泡芽的分析和周等离子体溶液的封装.
主要成果:
- 野生型洞穴蛋白的 Prokaryotic 表达诱导了含有聚合物洞穴蛋白的细胞质囊泡的形成.
- 这些异质洞穴 (h-洞穴) 在结构上与原生洞穴在尺寸和洞穴密度上相似.
- 低温电子断层扫描揭示了囊中叶素寡合体的潜在多面体排列.
结论:
- 洞穴可以在无细胞系统中自组装成洞穴状结构,独立于复杂的真核生物机械.
- 形成过程涉及细胞质膜的芽,形成不同的脂质域.
- 这些h-caveolae可以封装分子,并为有针对性的传递应用提供潜力.
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