细胞外的M. tuberculosis DNA通过激活宿主DNA感应途径来针对细菌进行自
Robert O Watson1, Paolo S Manzanillo, Jeffery S Cox
1Department of Microbiology and Immunology, Program in Microbial Pathogenesis and Host Defense, University of California, San Francisco, San Francisco, CA 94158, USA.
Cell
|August 21, 2012
概括
细胞使用自来消灭细胞质细菌. 这项研究揭示了Mycobacterium结核病DNA如何触发泛素标记,导致细菌传递到自细胞和对感染的关键天生的免疫力.
科学领域:
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
背景情况:
- 细胞使用自细胞来清除细胞质病原体.
- 自诱导限制了Mycobacterium tuberculosis在巨细胞中的复制,但其向机制和在自然感染中的作用尚不清楚.
研究的目的:
- 阐明了宿主细胞内Mycobacterium结核病自的向机制.
- 调查细胞核DNA感应和自在对M.结核病的天生的免疫力中的作用.
主要方法:
- 通过细菌ESX-1分泌系统研究了体透.
- 利用STING-依赖的细胞解质途径用于细菌的无处不在.
- 研究了p62,NDP52和TBK1在自细胞分泌中的作用.
- 评估了具有缺陷自途径的小鼠对M.结核病感染的敏感性.
主要成果:
- 细菌ESX-1分泌系统介导着体透,允许细胞质进入M.结核病.
- 通过STING-依赖途径对细胞外细菌DNA的识别对于细菌无处不在是必不可少的.
- 乌比基-自受体p62和NDP52,以及激酶TBK1,介导细菌向自体的传递.
- 缺乏将细菌传递到自的能力的小鼠对M.结核病感染表现出极度的敏感性.
结论:
- 揭示了DNA感知,先天免疫和自之间在对抗M.结核病时的意想不到的联系.
- 证明这种自途径在抵抗M.结核病感染方面发挥着关键作用.
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