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Updated: May 19, 2026

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
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Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Published on: November 22, 2014

作为蛋白质甲基化报告者的Se-adenosyl-L-selenomethionine辅因子类似物

Ian R Bothwell1, Kabirul Islam, Yuling Chen

  • 1Molecular Pharmacology and Chemistry Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10065, USA.

Journal of the American Chemical Society
|August 25, 2012
PubMed
概括

研究人员开发了一种新的S-adenosyl-L-methionine (SAM) 替代品ProSeAM,以有效报告甲基转移酶活性. 这种新的化学探针可以在各种细胞环境中进行更广泛的基质标记和内源蛋白甲基转移酶的分析.

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Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...