开发一个带有转化开关的化探针,用于检测基因素脱乙酶活性
Reisuke Baba1, Yuichiro Hori, Shin Mizukami
1Graduate School of Engineering, Osaka University, Suita, Osaka, 565-0871, Japan.
Journal of the American Chemical Society
|August 25, 2012
概括
研究人员开发了一种新的化探针,K4(Ac) -CCB,用于简单的,单步监测基因素脱乙酶 (HDAC) 活性. 这种工具有助于表观遗传研究和发现HDAC向药物.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 基因脱乙酶 (HDACs) 是调节基因表达,细胞循环和癌症发展的关键酶.
- HDACs是治疗药物开发的重要目标.
- 监测HDAC活动的简单有效方法是有限的.
研究的目的:
- 开发一种新的,简单和有效的化探针来检测HDAC活动.
- 建立一个单步程序来监测HDAC酶功能.
- 为了证明探针在评估HDAC抑制剂疗效方面的实用性.
主要方法:
- 开发一种化探针 (K4(Ac) -CCB),其中包括一个与乙-Lys和氨酸合剂的素H3.
- 利用自发的分子内转化以在HDAC活动时产生光信号.
- 在HDAC活性检测和抑制剂查的一步测试中应用探针.
主要成果:
- K4 (((Ac) -CCB探头通过光清晰地检测HDAC活动.
- 试验是一个简单的,一步骤的程序,促进快速评估.
- 该探针有效评估HDAC抑制剂的活性.
结论:
- 新型化探针K4(Ac) -CCB为HDAC活动监测提供了强大而简单的工具.
- 这种方法显著推进了表观遗传研究和针对HDAC的药物发现.
- 单步程序简化了酶活性和抑制剂查的评估.
更多相关视频
09:07Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
Published on: May 1, 2019
12:07Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
Published on: November 22, 2014
相关概念视频
03:31Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
02:07FISH - Fluorescent In-situ Hybridization
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...