百甲的内分泌物 菌体识别植物性细胞壁多糖的独特碳水化合物表位,具有高度亲和力和选择性
Kai-For Mo1, Xiuru Li, Huiqing Li
1Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, Georgia 30602, USA
Journal of the American Chemical Society
|September 1, 2012
概括
菌体内素通过细胞壁结合域 (CBD) 结合细菌细胞壁. 具体性来自于这些CBD如何识别寡糖类上的银河系化模式.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 结构生物学 结构生物学
背景情况:
- 菌体使用内素来降解酸糖,以释放后代.
- 恩多利辛具有细胞壁结合域 (CBDs),对基质特异性至关重要.
- 对于CBD对格朗阳性细菌细胞壁碳水化合物的特异性的分子基础尚不清楚.
研究的目的:
- 为了研究Endolysin特异性背后的分子机制.
- 合成和描述模仿Bacillus anthracis二级细胞壁多糖的寡糖.
- 确定内素CBD与这些合成的寡糖的结合亲和力.
主要方法:
- 一个具有不同银河系化模式的寡糖类库的化学合成.
- 使用直角保护组 (Fmoc,Nap,Lev,TDS) 进行精确的合成.
- 表面等离子体共振 (SPR) 用于测量内素 (PlyL,PlyG) 和寡糖之间的解离常数 (Kd).
主要成果:
- 银河化模式显著影响了内溶素的结合亲和力.
- 一种特定的寡糖与一个galactosyl部分在C-4的nonreducing GlcNAc表现出低微分子结合.
- 在细菌中,二次细胞壁多糖的结构变异,特别是银河系化,是很常见的.
结论:
- 恩多利辛对特定细菌物种的特异性可能是通过选择性结合到独特的银河化模式来调节的.
- 了解这些相互作用可以为开发有针对性的菌体治疗提供信息.
- 合成的寡糖库是研究宿主-菌体相互作用的宝贵工具.
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