自组装的生物活性β片纳米结构的结构和形状动态,这些纳米结构装饰着多价值RNA结合
Sanghun Han1, Donghun Kim, So-hee Han
1Translational Research Center for Protein Function Control and Department of Materials Science & Engineering, Yonsei University, Seoul 120-749, Korea.
Journal of the American Chemical Society
|September 4, 2012
概括
生物活性纳米结构表现出动态的外移动性和稳定的核心. 它们的结构响应环境变化,为设计响应性纳米材料提供了洞察力.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 生物物理学的生物物理.
背景情况:
- 了解纳米材料动力学对于开发可控制,量身定制的材料至关重要,特别是对于生物应用.
- 生物分子本质上是动态的,对外部刺激有反应,因此需要对纳米结构进行动态研究.
研究的目的:
- 研究自组装生物活性β片纳米结构的结构和构造动力学.
- 阐明生物活性外和β-丝带核心动态之间的关系.
主要方法:
- 电子磁共振 (EPR) 光谱学被用来研究动态.
- 具有跨β脊柱和生物活性外的模型纳米结构的特征.
主要成果:
- 中的生物活性显示出类似于孤立单体的移动性.
- 十字β脊柱外围的移动性低于表面显示的.
- 纤维动力学是独立于聚合长度的,外类首先对环境变化做出反应.
结论:
- 十字β脊柱表现出环境阻力,在发生重大干扰时突然解体.
- 这些发现提供了对β片纳米结构动态的全面理解.
- 这些结果对于设计各种应用的动态自组装纳米结构具有价值.
相关概念视频
Protein Folding
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Protein Structure Is Critical to Its Biological Function
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Overview
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Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
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Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
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