淋巴细胞通过整合素受体alpha 4 beta 1识别纤维素的替代拼接部分
1Center for Cancer Research, Department of Biology Massachusetts, Institute of Technology, Cambridge 02139.
Cell
|January 12, 1990
概括
WEHI 231淋巴细胞需要替代拼接的纤维素来扩散,特别是通过整合素α4β1与V25段结合. 这种相互作用突显出一种特定的细胞粘附机制.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 纤维蛋白 (FN) 是一种关键的细胞外基质蛋白,参与细胞粘附,迁移和分化.
- FN转录的替代拼接产生了具有独特功能性质的各种异型.
- 了解与FN异构体的细胞特异性相互作用对于阐明生物过程和疾病机制至关重要.
研究的目的:
- 为了确定负责WEHI 231淋巴细胞粘附的特定纤维素 (FN) 区域和细胞表面受体.
- 为了研究替代拼接在纤维内素介导细胞扩散中的作用.
- 描述淋巴细胞中整合素-纤维肌菌素相互作用的分子基础.
主要方法:
- 使用的纯化重组纤维素与或没有替代拼接的V区域.
- 采用V25 (CS-1) 段的合成来阻止细胞扩散.
- 使用合成定位了结合部位,并确定了涉及的整合蛋白.
- 使用对整合素α4的抗体来抑制细胞扩散.
主要成果:
- WEHI 231 淋巴细胞被证明仅在含有替代拼接的 V 区域 (V+ FN) 的纤维菌素上传播.
- 细胞在V+ FN上扩散被V25段的特异性抑制.
- 在V25区域内保存的10氨基酸段被确定为主要的结合部位.
- 鉴定出综合素α4β1是调解这种特定粘附的主要受体,与V25段结合.
结论:
- 整体素α4β1作为替代拼接细胞粘附部位 (V25/CS-1) 的特定纤维内素受体.
- 这种相互作用对于WEHI 231淋巴细胞粘附于特定的纤维素因子形式至关重要.
- 这些发现表明,整合素α4β1在各种细胞类型选择性粘附于不同纤维内素变异中起着重要的作用.
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