Rad51是Dmc1-介导的Dmc1-介导的关节分子形成的辅助因子
Veronica Cloud1, Yuen-Ling Chan, Jennifer Grubb
1Committee on Genetics, University of Chicago, Cummings Life Science Center, 920 East 58th Street, Chicago, IL 60637, USA.
Science (New York, N.Y.)
|September 8, 2012
概括
发芽的酵母半转化依赖Rad51和Dmc1蛋白质进行DNA重组. Rad51的联合分子活动是不可或缺的,而Dmc1是必不可少的,突出显示Dmc1在这个过程中的关键作用.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 介质重组对于介质过程中精确的染色体分离至关重要.
- 与ReCA相关的蛋白质Rad51和Dmc1对于发芽酵母的同类重组至关重要.
- 这两种蛋白质都在DNA上形成细丝,促进同质性搜索和链交换.
研究的目的:
- 调查Rad51和Dmc1在介质重组中的不同作用.
- 为了确定Rad51的联合分子 (JM) 形成活动是否对化至关重要.
- 在介质重组过程中阐明Rad51和Dmc1之间的功能关系.
主要方法:
- 使用了Rad51的功能分离突变,保留了光线形成,但缺乏JM活性.
- 引入了对应的突变到Dmc1.1.
- 进行生物化学测试以评估蛋白质相互作用和活动.
主要成果:
- 发现Rad51的联合分子形成活动对于介质重组是不可或缺的.
- 在Dmc1中发生的类似突变导致了中介性重组的显著缺陷.
- 生物化学证据表明,Rad51作为Dmc1辅助因子与Mei5-Sae3复合体一起起作用.
结论:
- Dmc1的联合分子形成活动是唯一负责介质重组的.
- 拉德51具有多功能作用,在线粒分裂过程中直接催化重组,在半分裂过程中通过Dmc1间接催化.
- 这项研究阐明了Rad51和Dmc1在复杂的介质重组过程中的专门功能.
相关概念视频
02:20Cohesins
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Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
02:15Condensins
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The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
02:02Meiosis II
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The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
03:09Meiosis I
Meiosis is the division of a diploid cell into haploid cells forming sperm and eggs in animals through differentiation. Meiosis I is the first stage of meiosis, where the genetic recombination of homologous chromosomes and the reduction of the ploidy level by half occurs.
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
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01:49Meiosis I
Meiosis is a carefully orchestrated set of cell divisions, the goal of which—in humans—is to produce haploid sperm or eggs, each containing half the number of chromosomes present in somatic cells elsewhere in the body. Meiosis I is the first such division, and involves several key steps, among them: condensation of replicated chromosomes in diploid cells; the pairing of homologous chromosomes and their exchange of information; and finally, the separation of homologous chromosomes by a...
03:09Meiosis I
Meiosis is the division of a diploid cell into haploid cells forming sperm and eggs in animals through differentiation. Meiosis I is the first stage of meiosis, where the genetic recombination of homologous chromosomes and the reduction of the ploidy level by half occurs.
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...