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两种长非编码RNA的转录调解了在芽酵母菌中发生性生殖的交配类型控制
Folkert J van Werven1, Gregor Neuert, Natalie Hendrick
1David H. Koch Institute for Integrative Cancer Research and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Cell
|September 11, 2012
概括
两种长非编码RNA (lncRNAs) 通过调节IME1基因来控制酵母细胞的发生. 它们的转录抑制了哈普洛伊德体中的胞形成,但对二倍体诱导至关重要,控制细胞命运决策.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 细胞生物学 细胞生物学
背景情况:
- 性繁殖依赖于游戏生成,这是一个关键的细胞命运决定.
- 在酵母菌 (Saccharomyces cerevisiae) 中,只有双倍体细胞 (MATa/α) 进行分泌,而不是双倍体细胞 (MATa或MATα).
- 交配类型的胞控制是关键的监管过程.
研究的目的:
- 调查长非编码RNAs (lncRNAs) 在配合类型控制类型的游戏生成中的作用.
- 阐明 lncRNAs 调节酵母体胞的分子机制.
主要方法:
- 在平分体和二分体酵母细胞中对lncRNA转录的分析.
- 研究lncRNA表达对IME1和IME4基因调节的影响.
- 染色体免疫沉以评估IME1促进体中的基因素修饰.
主要成果:
- 两种特定的lncRNAs,IRT1和一个反意义转录,被确定为交配类型控制的媒介.
- 在平体中,IRT1转录通过招募抑制性染色体修饰剂 (Set2,Set3复合物) 来抑制中央游戏生殖诱导物IME1.
- 干扰这些 lncRNAs 在平体中使胞形成,而它们在双体中的存在取消了它.
结论:
- 特定的lncRNAs的转录控制着酵母体中伴侣类型依赖的游戏生成调节.
- lncRNAs充当关键的调节者,确保正确的细胞命运决定性繁殖.
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