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Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
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Published on: September 15, 2017

可编程的水凝用于控制细胞捕获和释放,使用杂交的体和互补序列.

Zhaoyang Zhang1, Niancao Chen, Shihui Li

  • 1Department of Chemical, Materials, and Biomolecular Engineering, School of Engineering, University of Connecticut, Storrs, Connecticut 06269, United States.

Journal of the American Chemical Society
|September 14, 2012
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种可编程的水凝,用于控制细胞捕获和释放,使用阿普坦和补充序列. 这种方法可实现高效,生物相容的细胞分离和再生,用于各种应用.

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科学领域:

  • 生物材料科学 生物材料科学
  • 细胞生物学 细胞生物学
  • 再生医学是一种再生医学.

背景情况:

  • 调节细胞物质相互作用对于再生医学和细胞分离等应用至关重要.
  • 现有的方法可能缺乏精确的控制或生物相容性.

研究的目的:

  • 开发一种可编程的水凝系统,用于控制细胞捕获和释放.
  • 为了利用阿普坦和互补序列 (CSs) 进行动态细胞粘附调节.

主要方法:

  • 为特定的细胞结合而集成在水凝表面的工程aptamer.
  • 引入辅助序列 (CSs) 来触发体状态的变化.
  • 在引入CS时监测细胞粘附和释放动态.

主要成果:

  • 在混合状态下,aptamers促进稳定,细胞类型特定的捕获在水凝上.
  • 引入CSs会诱导胺转化,导致细胞快速释放.
  • 细胞捕获和释放过程是生物相容的,保留了细胞和水凝的完整性.

结论:

  • 开发的可编程水凝为控制细胞分离提供了一个新的,生物相容的平台.
  • 该系统的可再生性质允许细胞捕获和释放的重复循环.
  • 这项技术对再生医学及其他领域的先进应用具有前景.