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Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
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用生物对等化学物质探测菌根菌体三体

Benjamin M Swarts1, Cynthia M Holsclaw, John C Jewett

  • 1Department of Chemistry, University of California, Berkeley, California 94720, USA.

Journal of the American Chemical Society
|September 18, 2012
PubMed
概括

菌根菌利用三糖作为细胞壁的组成部分. 研究人员开发了亚化物修饰的三糖 (TreAz) 类似物来标记和可视化这些糖脂,有助于真菌细菌研究.

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...