人类黑色素瘤细胞系的瘤起源性通过引入人类染色体6进行控制
J M Trent1, E J Stanbridge, H L McBride
1University of Arizona, Arizona Cancer Center, Tucson 85724.
Science (New York, N.Y.)
|February 2, 1990
概括
染色体6上的基因可能控制人类黑色素瘤的生长. 将6号染色体引入黑色素瘤细胞减少了瘤的形成,而其去除则恢复了瘤,这表明6号染色体.
科学领域:
- 在瘤学瘤学.
- 人类遗传学 人类遗传学
- 癌症生物学 癌症生物学
背景情况:
- 染色体带分析揭示了人类恶性黑色素瘤6号染色体的非随机变化.
- 了解特定染色体异常的作用对于破译黑色素瘤发病因子至关重要.
研究的目的:
- 研究人类黑色素瘤6号染色体异常的功能相关性.
- 为了确定6号染色体是否含有抑制黑色素瘤瘤形成的基因.
主要方法:
- 用微细胞融合引入正常的6号染色体到人类黑色素瘤细胞系中.
- 结果的微细胞杂交体的表型性质和瘤性分析 in vitro 和 in vivo.
- 在引入或丢失6号染色体后,对裸体小鼠的瘤形成的评估.
主要成果:
- 在裸体小鼠中,引入了正常染色体6的黑色素瘤细胞系显示出改变的表型特性和瘤形成能力的显著减少.
- 混合细胞中引入的6号染色体的丢失导致瘤发生率的逆转,恢复形成瘤的能力.
- 单独引入对照选择性标记物 (psv2neo) 并没有影响瘤发生性,这表明该效应特定于染色体6.
结论:
- 这些发现强烈表明,位于6号染色体上的一个或多个瘤抑制基因在控制人类黑色素瘤恶性表型方面发挥着关键作用.
- 染色体6异常可能通过非激活这些关键基因,导致黑色素瘤的发展和进展.
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