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抗Fab吸收剂用于保护病毒免受中和抗体的影响

Darija Muharemagic¹, Mahmoud Labib, Shahrokh M Ghobadloo

¹Department of Chemistry, University of Ottawa, 10 Marie Curie, Ottawa, Ontario K1N 6N5, Canada.

Journal of the American Chemical Society

|September 29, 2012

概括

研究人员开发了DNA吸附体,以屏蔽型病毒免受中和抗体的影响,从而提高它们的有效性. 这些胺基改善了体内生存率,并使得病毒屏蔽和水平的精确量化成为可能.

科学领域:

生物技术是生物技术.
免疫学免疫学免疫学
纳米技术纳米技术

背景情况:

瘤病毒通过选择性向瘤细胞显示出癌症治疗的前景.
经过重复的病毒注射后产生的中和抗体 (nAbs) 可以降低治疗疗效.
开发克服nAbs的策略对于有效的体内性病毒疗法至关重要.

研究的目的:

选择针对抗囊性口腔炎病毒 (VSV) 抗体的抗原结合片段 (Fab) 的DNA吸收体.
评估这些体的能力,以屏蔽VSV从nAbs和增强其体内生存能力.
开发一种新型的电化学免疫传感器,用于量化阿巴胺介导屏蔽和VSV水平.

主要方法:

使用流细胞计和电化学免疫传感,对抗异状口腔炎病毒 (VSV) 多克隆抗体的Fab区域进行DNA吸管的选择.
在基于细胞的感染试验中评估阿普坦胺屏蔽效率.
开发一种双功能无标签电化学免疫传感器,使用电化学阻抗光谱技术进行VSV量化.

主要成果:

一些精选的阿普坦克克隆表明,VSV对中和抗体的屏蔽率超过50%.
开发的电化学免疫传感器准确地量化了VSV颗粒在线性范围的5 × 10 ((4) 到5 × 10 ((6) PFU mL ((-1) 之间,检测极限为10 ((4) PFU mL ((-1).
胺体显示出增强VSV传递的潜力,而不会损害患者的免疫系统.

结论:

DNA 体可以有效地保护像VSV这样的型病毒免受中和抗体的影响.
这种由阿巴胺介导的屏蔽策略有望提高瘤解毒性病毒疗法的体内疗效.
一种新的电化学免疫传感器提供了一种灵敏且无标签的方法来监测VSV水平和阿帕特胺介导屏蔽.

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