相关实验视频
Updated: May 18, 2026

09:20
CRISPR-Mediated Reorganization of Chromatin Loop Structure
Published on: September 14, 2018
基因循环增强了转录的方向性
Sue Mei Tan-Wong1, Judith B Zaugg, Jurgi Camblong
1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
概括
由积极转录的mRNA基因形成的基因循环限制了非编码RNA (ncRNA) 合成. 不激活Ssu72蛋白或基因循环会增加不同的ncRNA的产生,强制执行转录的方向性.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 基因规则 基因规则
背景情况:
- 细胞基因组经历广泛的转录,产生传递 RNA (mRNA) 和非编码 RNA (ncRNA).
- 非编码RNA通常来自双向促进体,导致转录在相反的方向.
- 了解差异转录的调节对于理解基因组表达至关重要.
研究的目的:
- 研究基因循环构造在调节异性转录中的作用.
- 确定Ssu72蛋白对促进子相关ncRNAs的合成的影响.
- 阐明活跃转录的基因如何影响转录的方向性.
主要方法:
- 在积极转录的mRNA基因中基因循环形成的分析.
- 研究Ssu72蛋白在转录调节中的功能.
- 在Ssu72无活化或基因突变时评估Ssu72受限转录 (SRT) 的合成.
主要成果:
- 活跃转录的mRNA基因采用一种基因循环构造,限制分歧的ncRNA转录.
- 基因循环形成的关键因素Ssu72蛋白的失活显著增加SRT合成.
- 通过突变破坏个体基因循环也会导致增强的SRT产生.
- 基因循环构造强制执行双向促进体的转录方向性.
结论:
- 基因循环形成是控制分歧转录和保持转录方向性的关键机制.
- Ssu72蛋白在建立和维持基因循环中发挥着至关重要的作用,从而抑制了ncRNA合成.
- 这些发现揭示了一种新的基因调节层,确保了编码和非编码RNAs的精确表达.
相关概念视频
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Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
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Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cooperative Binding of Transcription Regulators
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
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