在多个未培养的细菌群中发酵,和硫代谢
Kelly C Wrighton1, Brian C Thomas, Itai Sharon
1Department of Earth and Planetary Science, University of California, Berkeley, Berkeley, CA 94720, USA.
概括
研究人员发现了未培养的无氧细菌 (BD1-5,OP11,OD1) 和一个新的血统 (PER) 的代谢功能. 这些细菌发酵碳,可能在生产和硫循环中发挥关键作用.
科学领域:
- 微生物学 微生物学
- 转基因组学是指转基因组学.
- 生物地质化学生物地质化学
背景情况:
- 细菌族BD1-5,OP11和OD1的成员在无氧环境中很常见,但由于种植和基因组数据有限,它们的代谢作用尚不清楚.
- 了解这些细菌的新陈代谢对于理解无氧生态系统功能至关重要.
研究的目的:
- 阐明BD1-5,OP11和OD1.1类非培养细菌的代谢途径和生态作用.
- 描述一种新发现的细菌系 (PER) 以及其代谢能力.
主要方法:
- 49个细菌基因组的部分或近乎完整的培养独立的恢复从一个乙酸盐修改的含水层.
- 基因组分析以预测代谢功能,包括发酵,碳固定和节能途径.
主要成果:
- 所有分析的细菌都被预测为非呼吸性厌氧生物,主要利用发酵.
- 三组拥有一种新型的古生物学类混合体 - - 核糖-1,5-双酸碳氧化酶-氧化酶 (RuBisCO),用于与AMP救援相结合的二氧化碳固定.
- 发现OD1族的成员可以减少硫,并可能利用古老类型的化酶进行质子送.
- 在六种研究的生物体中,UGA停止编码子被翻译为酸盐.
- 确定了PER血统,并阐明了其代谢特征.
结论:
- 这些发现揭示了无氧细菌中以前未知的代谢策略,包括细菌中一种新的RuBisCO通路.
- 研究的细菌可能对的生产,硫循环和反抗性沉积碳的发酵作出了重大贡献.
- 这项研究扩大了我们对微生物暗物质及其在无氧环境中的作用的理解.
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