Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Genetically targeted photocatalytic organic dyes for spatiotemporally controlled organic synthesis in specific living cells.

Nature chemistry·2026

Same author

Tumor immune cell targeting chimeras reprogram tumor-associated macrophages.

Nature chemical biology·2026

Same author

Iterative Bump-and-Hole Engineering Creates a Bioorthogonal Reporter for <i>N</i>-Acetylglucosaminyltransferase I.

Journal of the American Chemical Society·2026

Same author

Mucin-binding protein shuttles enable delivery of brain-targeted therapeutics.

bioRxiv : the preprint server for biology·2026

Same author

Iterative Bump-and-hole engineering creates a bioorthogonal reporter for <math><mi>N</mi></math> -acetylglucosaminyltransferase I.

bioRxiv : the preprint server for biology·2026

Same author

The protease cathepsin K can debulk the cancer glycocalyx.

The Journal of biological chemistry·2026

Search research articles

相关实验视频

Updated: May 18, 2026

Autofluorescence Imaging to Evaluate Cellular Metabolism

Autofluorescence Imaging to Evaluate Cellular Metabolism

Published on: November 15, 2021

用于生物成像的化酸化素.

Peyton Shieh¹, Matthew J Hangauer, Carolyn R Bertozzi

¹Department of Chemistry, University of California, Berkeley, California 94720, USA.

Journal of the American Chemical Society

|October 3, 2012

概括

此摘要是机器生成的。

研究人员开发了用于生物对等成像的新型化酸探针. 这些探针在反应时显示增强的光,使细胞中的蛋白质和糖蛋白可以在不洗的情况下进行选择性标记.

更多相关视频

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Published on: January 5, 2015

相关实验视频

Last Updated: May 18, 2026

Autofluorescence Imaging to Evaluate Cellular Metabolism

Autofluorescence Imaging to Evaluate Cellular Metabolism

Published on: November 15, 2021

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Published on: January 5, 2015

科学领域:

化学生物学化学生物学
有机化学有机化学
生物医学成像技术生物医学成像技术

背景情况:

生物对等化学反应使生物分子在复杂的生物系统中的特定标记成为可能.
在反应时增加光的化探针是"不洗"成像的理想选择,减少背景噪声.
在生物对等激活后开发具有可预测光增强的探测器仍然是一个挑战.

研究的目的:

理性地设计和合成由生物对等循环添加反应激活的新型化酸探针.
为了识别与基因反应时显著增强光的亚齐多素衍生物.
为了证明这些探头在体外和细胞内无洗条件下标记生物分子的实用性.

主要方法:

密度函数理论 (DFT) 计算,以预测亚酸衍生物的光增强.
在模型反应中合成和实验验证四种亚齐多化衍生物.
在体外对基因功能化的蛋白质进行标记,并在细胞内对糖蛋白进行局部成像.

主要成果:

DFT计算指导了有前途的亚齐多化衍生品的识别.
四种合成衍生物在与基因 (Cu催化或无Cu) 反应时显示光增强.
一种4-azidonaphthylfluorescein模拟物证明了蛋白质和糖蛋白的选择性标记,使细胞可在不洗的情况下成像.

结论:

建立了一个平台,以合理设计用于生物直角成像的化酸探针.
在无洗条件下,证明了这些探针在选择性生物分子标记和细胞成像方面的成功应用.
强调了为各种生物成像应用量身定制这些探测器的光谱特性的潜力.