雨,风和触摸诱导的表达卡尔莫杜林和卡尔莫杜林相关基因在阿拉比多普西斯
1Department of Biochemistry, Beckman Center, Stanford University, California 94305.
Cell
|February 9, 1990
概括
阿拉比多普西斯植物通过改变基因表达和生长来响应触摸和其他刺激. 这项研究表明,离子和模素是植物感知和反应环境变化的关键因素.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 植物中的环境传感.
背景情况:
- 阿拉比多普西斯塔利亚纳在应对各种环境刺激时表现出受调节的基因表达.
- 触摸和伤害等机械刺激,以及诸如喷水,风和黑暗等非生物因素,在植物中触发反应.
- 触摸的发育反应Thigmomorphogenesis导致Arabidopsis的树枝和螺栓较短.
研究的目的:
- 研究植物对环境刺激的反应背后的分子机制.
- 为了识别和表征涉及到触摸诱导反应的基因在Arabidopsis.
- 探索离子和calmodulin在信号传导途径中的作用.
主要方法:
- 在Arabidopsis中触摸诱导 (TCH) 基因表达的分析.
- 在各种刺激后对mRNA水平的量化.
- 对TCH基因产物进行DNA测序和氨基酸身份比较.
主要成果:
- 至少有四个触摸诱导 (TCH) 基因受到刺激的调节,包括触摸,喷水,风和黑暗.
- TCH基因的mRNA水平可以在10-30分钟的刺激内增加100倍.
- TCH 1的cDNA编码了一个类似于calmodulin的蛋白质,其中TCH 2和TCH 3与calmodulin具有显著的氨基酸相同性.
结论:
- 与calmodulin相关的TCH基因家族的调节表明离子和calmodulin在环境信号传导中的作用.
- 植物拥有复杂的机制来感知和响应环境线索.
- 这些发现有助于理解植物感官生物学和适应.
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