摩托蛋白组合的拉战,通过可编程DNA原木脚手架揭示了这一点
N D Derr1, B S Goodman, R Jungmann
1Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
Science (New York, N.Y.)
|October 16, 2012
概括
合成DNA原始货物控制电机组合,揭示电机号码对速度的影响最小,但相反的极性电机参与可解决的拉战. 这有助于我们更好地理解细胞内运输协调.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 分子电机分子电机
背景情况:
- 细胞质的丁氨酸和丁氨酸-1是微管电机,其相反的极性对于细胞内运输至关重要.
- 双向货物流动是由电机组合产生的,但确切的空间和时间分类发生.
- 协调这些动力组合的机制仍然不完全理解.
研究的目的:
- 研究控制微管基电机组合的协调机制.
- 开发一种可控制的合成货物系统,用于实验室研究机动组合动力学.
主要方法:
- 使用3D DNA原创构造了一个可编程的合成货物.
- 附有不同数量的DNA寡核酸结合电机 (dynein和kinesin-1),具有受控的类型,数量,间距和方向.
- 在试管体内分析了运动组合行为.
主要成果:
- 在同一极性电机 (1-7 电机) 组合中,增加电机数对方向速度的影响最小.
- 两极相反的电机组合表现出一种"拉战"的行为.
- 这种拉斗争可以通过脱离一个运动物种来解决.
结论:
- 发动机号码不是相同极性的组合中速度的主要决定因素.
- 两极相反的电机相互作用创造了一个可解决的拉战,这对于货物运输监管至关重要.
- 这种合成系统为剖析细胞过程中复杂的运动协调提供了一个平台.
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