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DAXX包裹着一个用于H3.3特定识别的素H3.3-H4二元体

Simon J Elsässer¹, Hongda Huang, Peter W Lewis

¹Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, New York 10065, USA.

|October 19, 2012

概括

DAXX 基因组辅助器特意识别了基因组变异 H3.3.3. 结构和功能研究揭示了DAXX如何结合H3.3-H4二元体,澄清了H3.3识别特异性.

科学领域:

分子生物学分子生物学
结构生物学结构生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

在染色体组装过程中防止不适当的基因素相互作用,基因素陪伴者至关重要.
达克斯是一种甲菌组织组织伴侣,专门结合组织组织变体H3.3.3.
了解H3.3的识别是阐明色素动态的关键.

研究的目的:

为了确定DAXX陪伴者对基因素H3.3识别的结构基础.
阐明DAXX-H3.3特异性背后的分子机制.
在体外和体内研究DAXX-H3.3相互作用的功能影响.

主要方法:

用H3.3-H4二聚体和突变物复杂的DAXX基因素结合域的X射线晶体学.
在体外结合测定.
在体内功能性研究.

主要成果:

DAXX包裹在H3.3-H4二元体周围,诱导组织组织的结构变化.
DAXX利用扩展的α螺旋形状来破坏关键的交互点.
在H3.3 (Gly90) 和DAXX (Glu225) 中特定的残留物被确定为识别特异性的关键.

结论:

这项研究为DAXX. 3对H3.3变体的识别提供了原子层面的见解.
DAXX充当一个专业的陪伴者,防止乱交的H3.3相互作用.
这些发现有助于我们更好地了解基因组变异沉积和染色质调节.

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