一个发光和生物相容的PhotoCORM
Agustin E Pierri1, Alessia Pallaoro, Guang Wu
1Department of Chemistry and Biochemistry, University of California, Santa Barbara, California 93106, USA.
Journal of the American Chemical Society
|October 20, 2012
概括
这项研究引入了一种发光I复合物,fac-[Re(bpy) ((3) ((CO) ((3) ((thp) ]+,作为光活性一氧化碳释放分子 (photoCORM). 它是细胞透的,无毒的,其CO释放可以在细胞内实时跟踪.
科学领域:
- 无机化学 无机化学
- 摄影化学的使用.
- 细胞生物学 细胞生物学
背景情况:
- (I) 复合物因其发光和光活性特性而被探索.
- 一氧化碳释放分子 (CORM) 有治疗潜力.
- 开发光激活的CORM (photoCORM) 允许对CO释放进行时空控制.
研究的目的:
- 为了合成和表征一种水溶性 (I) 复合物,面-[Re (bpy) (III) (CO) (III) (thp) ]+,作为一个光CORM.
- 评估其光物理性质,稳定性,细胞吸收和细胞毒性.
- 为了证明其在追踪癌细胞内CO释放中的实用性.
主要方法:
- (I) 复合物的合成和表征.
- 光物理测量 (发光).
- 使用PPC-1细胞进行细胞吸收研究.
- 同焦点光显微镜可视化细胞内CO释放.
主要成果:
- 复杂的fac-[Re(bpy) ((3) ((CO) ((3) ((thp) ((+) 是强烈的发光和光活性,在照射时释放CO.
- 它在水性介质中稳定,被PPC-1细胞吸收,并且不表现出细胞毒性.
- 发光光电产品允许实时跟踪细胞内CO释放的情况.
结论:
- (I) 复合物是一个有前途的光CORM,具有潜在的治疗应用.
- 它以受控的方式释放二氧化碳并被细胞内可视化的能力得到了证明.
- 这项工作突出了发光复合物的光药学潜力.
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