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Updated: May 17, 2026

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
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基于功能化可溶性纳米聚合物的蛋白质表达和酸化的多重复合量化

Li Pan1, Anton Iliuk, Shuai Yu

  • 1Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA.

Journal of the American Chemical Society
|October 24, 2012
PubMed
概括

这项研究引入了一种新型纳米聚合物pIMAGO,用于同时测量蛋白质酸化和表达. 这一创新使得通过区分酸化和蛋白质水平变化,可以进行精确的细胞信号分析.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...