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针对超分辨率光成像的化Cy3-Cy5亲和探针的合成

Na Fu1, Yijia Xiong, Thomas C Squier

  • 1Biological Sciences Division, Fundamental Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington 99352, United States.

Journal of the American Chemical Society
|November 3, 2012
PubMed
概括

研究人员开发了一种新的可光开关的光探针,用于精确标记蛋白质. 这允许在蛋白质复合体的自然细胞环境中进行先进的现场成像.

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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.