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Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time
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在二叶酸还原酶中,有两种烯氨形成了一个催化剂,使得蛋白质动态的研究成为可能

Shengxi Chen1, Lin Wang, Nour Eddine Fahmi

  • 1Center for BioEnergetics, Biodesign Institute, and Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.

Journal of the American Chemical Society
|November 3, 2012
PubMed
概括

研究人员用基组对二叶酸还原酶 (DHFR) 进行了工程设计,以观察脱酶的形成,揭示了与酶反应期间产品释放相关的蛋白质构造变化.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...