在SUMO途径中蛋白质组的修饰和协同作用,如DNA修复中所示
Ivan Psakhye1, Stefan Jentsch1
1Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
Cell
|November 6, 2012
概括
蛋白质修饰的SUMOylation同时对多个DNA修复蛋白质产生协同作用. 这种集体修改,而不是个别实例,对于高效的DNA双链断裂修复至关重要,突出了群体向机制.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- SUMOylation (Small Ubiquitin-like Modifier) 是一种影响众多细胞过程的翻译后修饰.
- 虽然SUMO途径突变显示出强大的表型,但基板上的单个SUMOylation位点的功能意义往往不清楚.
- 缺陷SUMOylation的突变通常缺乏明显的表型后果,这表明复杂的调节作用.
研究的目的:
- 研究SUMOylation在DNA双链断裂修复中的作用.
- 为了确定SUMOylation是否对某一途径内的单个蛋白质或蛋白质组起作用.
- 阐明DNA修复过程中SUMOylation的机制和特异性.
主要方法:
- 利用DNA双链断裂修复作为一个模型系统.
- 研究了由DNA损伤引发的SUMOylation动态.
- 分析了多位 SUMOylation 对蛋白相互作用和同源重组效率的影响.
主要成果:
- DNA损伤诱导了一波SUMOylation波,影响DNA修复途径中的多种蛋白质.
- 由单链DNA触发的与DNA结合的SUMO酶介导了这种同时修饰.
- 在几个修复蛋白中完全消除SUMOylation显著损害同源重组并减缓DNA修复.
结论:
- SUMOylation 对一组蛋白质具有协同作用,个别的修改对有效的修复有添加性的贡献.
- SUMOylation可能针对蛋白质复合体或途径,而不是孤立的蛋白质.
- 局部化的SUMOylation酶和特定的触发器确保路径的特异性.
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