针对目标探头设计的PEG-化屏蔽方法
Yanyan Guo1, Hushan Yuan, William L Rice
1Center for Translational Nuclear Medicine and Molecular Imaging, Massachusetts General Hospital, 149 13th Street, Charlestown, Massachusetts 02129, USA.
Journal of the American Chemical Society
|November 10, 2012
概括
我们开发了PEG-化屏蔽,这是一种改进光探针的新方法. 这种技术提高了探针的亮度,并减少了不必要的相互作用,以获得更好的体外和体外成像.
科学领域:
- 生物医学工程 生物医学工程
- 分子成像学分子成像学
- 材料科学 材料科学 材料科学
背景情况:
- 光探针对于生物成像非常重要.
- 色素可以与生物分子相互作用,导致信号减少和非特异性结合.
- 目前的探针设计通常涉及合成新的色素,这是复杂的.
研究的目的:
- 为设计先进的光探测器引入和验证一种新的"PEG-化屏蔽"策略.
- 为了证明聚乙烯糖醇 (PEG) 可以通过屏蔽色素来提高探针性能.
- 探索这种屏蔽方法对向分子成像的实用性.
主要方法:
- 合成带有或没有5kDa的PEG功能组附着在色素上的类探针.
- 使用吸收光谱和光激活细胞分类 (FACS) 进行体外表征,以评估自我灭和细胞相互作用.
- 在体内使用表面光成像和用 (111) 标记RGD探针对整体的向成像进行体内评估.
主要成果:
- 在体外,PEGylation显著降低了色的自我灭.
- 与没有屏蔽的探针相比,PEG屏蔽的探针对细胞的非特异性结合减少.
- 在体内,PEG屏蔽通过阻断与生物分子的相互作用来最大限度地减少探针的保留.
- 通过使用PEG屏蔽的RGD探针实现了成功的向整合蛋白成像.
结论:
- PEG-化屏蔽是一种有效的策略,可以提高光探测器的量子产量,并最大限度地减少不必要的相互作用.
- 这种方法为开发有针对性的近红外光探测器提供了对de novo色合成的多功能替代方案.
- 该方法对分子成像中的主动和被动准应用都有希望.
相关概念视频
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