通过部分脱硫/减硫策略,对高度循环的兰地生物酸痛杀剂A进行结构性表征
Christopher T Lohans1, Zedu Huang, Marco J van Belkum
1Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2.
Journal of the American Chemical Society
|November 22, 2012
概括
研究人员发现了paenicidin A,这是一个来自Paenibacillus polymyxa的新型兰地生物. 这种高度循环的抗微生物具有六个 thioether 交叉链,为食品保存和治疗提供了潜力.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 类化学 类化学
背景情况:
- 抗菌素是细菌抗微生物,具有乙烯交叉链接,对食品保存和治疗有价值.
- 帕尼巴西勒斯多米克萨NRRL B-30509产生已知的抗生素和一种新型的兰西生物,帕尼西丁A.
- 此前从该菌株获得细菌素SRCAM 602的报道是毫无根据的.
研究的目的:
- 为了描述由Paenibacillus polymyxa.生产的新型兰地生物 paenicidin A 的特征.
- 为了阐明帕尼西丁A的复杂的乙烯交叉链接连接性,A.
- 评估帕尼西丁A的结构新性和潜在应用.
主要方法:
- 从Paenibacillus polymyxa中分离和净化帕尼西丁A NRRL B-30509.
- 新的部分脱硫/减硫策略用于thioether债券分析.
- 串联质谱 (MS/MS) 用于对交叉链接连接的结构阐明.
主要成果:
- 鉴定和结构性特征的帕尼西丁A,一个新型的lantibiotic.
- 帕尼西丁A含有六个氨酸和甲基氨酸环,其中有三个相互锁定的环.
- 一种新方法成功确定了乙烯交叉链接连接性,克服了NMR的局限性.
结论:
- 帕尼西丁A是一种结构独特且高度循环的兰地生物.
- 新的脱硫/减少MS/MS策略对复杂的lantibiotic结构是有效的.
- 帕尼西丁A的独特结构要求对抗微生物应用进行进一步的研究.
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