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Updated: May 12, 2026

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
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通过酸化核糖体捕获激活神经元的分子分析

Zachary A Knight1, Keith Tan, Kivanc Birsoy

  • 1Laboratory of Molecular Genetics, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. zachary.knight@ucsf.edu

Cell
|November 27, 2012
PubMed
概括

研究人员通过捕获酸化核糖体来确定激活的神经元. 这种方法揭示了下丘脑中控制食行为的特定神经元,包括加拉林和普罗迪诺芬神经元.

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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...