相关实验视频

Updated: Feb 3, 2026

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

MHC基因剂量和等位基因变异对T细胞受体选择的影响

L J Berg¹, G D Frank, M M Davis

¹Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305.

|March 23, 1990

概括

对T细胞受体的胸膜选择效率取决于主要基因相容性复杂分子密度. 这些分子中的特定结位变异影响T细胞选择结果.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
发展生物学发展生物学

背景情况:

胸膜选择对于T细胞谱的发展至关重要.
主体组织相容性复合体 (MHC) 分子向T细胞呈现.
与MHC-复合体的T细胞受体 (TCR) 相互作用介导了选择.

研究的目的:

调查MHC分子密度在胸膜阳性选择中的作用.
确定MHC等位基变异如何影响TCR选择效率.
探索结位对T细胞选择的影响.

主要方法:

使用T细胞受体转基因小鼠模型.
根据MHC分子表达水平评估T细胞选择效率.
分析了具有不同结位的MHC等位基变异.

主要成果:

更高的MHC分子 (I-Ek/b) 密度增强的转基因TCRαββ异构体选择.
MHC变种 (E alpha k:E beta b,E alpha k:E beta s,I-Ed) 显示选择效率降低或没有选择效率.
在MHC结位的差异,而不是TCR接触残留,与选择效率相关.
所有测试的IE变体都选择了β链转基因小鼠中的CD4+T细胞.

结论:

胸膜阳性选择具有受到MHC密度影响的随机成分.
特定的-MHC相互作用,特别是在结合部位内,对于TCR选择至关重要.
大致上,MHC分子结构和呈现显著地塑造了正在发展的T细胞谱.

更多相关视频

Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining

Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining

Published on: December 23, 2016

Examination of Thymic Positive and Negative Selection by Flow Cytometry

Examination of Thymic Positive and Negative Selection by Flow Cytometry

Published on: October 8, 2012

相关概念视频

Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8+ T Cells During Tumorigenesis

Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8⁺ T Cells During Tumorigenesis

The experimental design presented here provides a useful reproductive model for the studies of antigen-specific CD8+ T cells during lymph node (LN) metastasis, which excludes the perturbation of bystander CD8+ T...

A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice

A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice

Clonal expansion is a key feature of antigen-specific T cell response. However, the cell cycle of antigen-responding T cells has been poorly investigated, partly because of technical limitations. We describe a flow cytometric method to analyze clonally expanding antigen-specific CD8 T cells in spleen and lymph nodes of vaccinated...

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8⁺ T Cell Responses

We describe here a flow cytometry-based in vivo killing assay that enables examination of immunodominance in cytotoxic T lymphocyte (CTL) responses to a model tumor antigen. We provide examples of how this elegant assay may be employed for mechanistic studies and for drug efficacy...

In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

Here, we describe a method that combines in situ MHC-tetramer staining with immunohistochemistry to determine localization, phenotype, and quantity of antigen-specific T cells in tissues. This protocol is used to determine the spatial and phenotypic characteristics of antigen-specific CD8 T cells relative to other cell type and structures in tissues.

Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining

Analysis of Simian Immunodeficiency Virus-specific CD8⁺ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining

Here, we present an optimized protocol for enumerating and characterizing rhesus macaque CD8+ T cells against the AIDS virus. This article is useful not only to the field of HIV immunology, but also to other areas of biomedical research where CD8+ T cell responses are known to affect disease...

Examination of Thymic Positive and Negative Selection by Flow Cytometry

Examination of Thymic Positive and Negative Selection by Flow Cytometry

We present a flow cytometry-based method to examine T cell development in vivo using genetically manipulated mice on a wildtype or T cell receptor transgenic...