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相关概念视频

Cytomegalovirus Disease01:27

Cytomegalovirus Disease

Cytomegalovirus (CMV) disease is caused by human cytomegalovirus, a double-stranded DNA virus of the Herpesviridae family. While primary CMV infection is often asymptomatic in immunocompetent individuals, the virus can cause severe disease in neonates and immunocompromised patients. CMV is the most common cause of congenital viral infection in the United States, and a major pathogen in solid organ and hematopoietic stem cell transplant recipients.CMV is transmitted via bodily fluids, sexual...
Human Virome01:26

Human Virome

The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...

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相关实验视频

Updated: May 16, 2026

qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
08:08

qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue

Published on: July 9, 2014

解码人类细胞巨型病毒.

Noam Stern-Ginossar1, Ben Weisburd, Annette Michalski

  • 1Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisico, CA 94158, USA.

Science (New York, N.Y.)
|November 28, 2012
PubMed
概括
此摘要是机器生成的。

研究人员发现了人类细胞巨乳病毒 (HCMV) 基因组的新细节,揭示了数百个以前未知的蛋白质编码区域. 这一发现突出了病毒的存在.

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Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
11:28

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Published on: October 7, 2011

相关实验视频

Last Updated: May 16, 2026

qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
08:08

qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue

Published on: July 9, 2014

Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates
05:53

Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates

Published on: July 6, 2013

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
11:28

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Published on: October 7, 2011

科学领域:

  • 病毒学 病毒学
  • 基因组学就是基因组学.
  • 分子生物学分子生物学

背景情况:

  • 人类细胞巨乳病毒 (HCMV) 基因组尽管在20年前被测序了,但它对其蛋白质编码潜力仍然有许多.
  • 了解病毒基因表达的全部范围对于理解病毒复制和病变产生至关重要.

研究的目的:

  • 通过实验来定义HCMV翻译产品的完整套.
  • 为了研究这些病毒蛋白的时间表达模式.
  • 在HCMV基因组中发现新的编码元素.

主要方法:

  • 利用核糖体概况来捕获HCMV转录组的活跃翻译区域.
  • 采用转录分析来识别和表征病毒RNA物种.
  • 应用质谱测量以确认预测的病毒多的存在.

主要成果:

  • 在HCMV基因组中确定了数百个以前未被发现的开放阅读框架 (ORF).
  • 通过质谱分析证实了这些新型ORF的子集.
  • 证明受监管的替代转录开始站点被广泛使用.
  • 表明这些替代起点能够精确控制蛋白质表达的时间.
  • 揭示了一个单一的基因组位点可以产生多个不同的多.

结论:

  • HCMV基因组具有比以前估计的更大的编码能力.
  • 规范的替代转录开始站点使用是推动病毒编码复杂性的关键机制.
  • 这种复杂性允许复杂的时间调节病毒蛋白生产.