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Updated: May 16, 2026
12:08
Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
Published on: May 22, 2013
实时检测端粒酶活性,使用端粒重复试验的指数级异热放大
1Department of Chemistry, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, United States.
Journal of the American Chemical Society
|November 29, 2012
概括
一种名为指数异热放大端粒重复的新分析 (EXPIATR) 可以快速轻松地检测癌细胞中的端粒酶活性. 这种敏感的方法测量端粒长度,对癌症研究和衰老研究很有用.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 癌症研究 癌症研究
背景情况:
- 端粒和端粒酶在癌症和人类衰老中起着至关重要的作用.
- 精确测量端粒酶活性对于生物研究至关重要.
研究的目的:
- 为测量端粒酶活性引入一种新,敏感,简单和可靠的体外试验法.
- 开发一种超快的检测方法,避免昂贵的热循环.
主要方法:
- 开发了高指数异热放大端粒重复 (EXPIATR) 试验.
- 使用核酸同热放大器的战略设计.
- 评估细胞提取物中的端粒酶活性.
主要成果:
- 该EXPIATR试验提供了对端粒酶活性的敏感和可靠的测量.
- 该试验实现了超快的检测,结果大约在25分钟内得到.
- 它可以检测到相当于单个HeLa癌细胞的端粒酶活性.
结论:
- EXPIATR是一种强大的新工具,用于研究端粒酶.
- 测试的简单性和速度使其对癌症和衰老研究具有价值.
- 埃克斯皮亚特提供了一个替代传统的,更昂贵的方法,用于端粒酶检测.
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06:38Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction
Published on: May 3, 2019
08:26Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
Published on: June 12, 2018
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In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.