瘤细胞表现出细胞周期的基因组素基因促进因子HiNF-D的放松调节
J Holthuis1, T A Owen, A J van Wijnen
1Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655.
Science (New York, N.Y.)
|March 23, 1990
概括
细胞周期调节的基因转录对正常细胞生长至关重要. 在癌细胞中异常的HiNF-D结合活性有助于不受控制的增殖和生长调节的丧失.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
背景情况:
- 细胞周期调节的基因表达对于正常的细胞生长和发育至关重要.
- 失去严格的细胞生长控制是转变,瘤表型的标志.
- 在S阶段的质蛋白合成对于DNA复制和染色质包装至关重要.
研究的目的:
- 为了研究HiNF-D结合活性在细胞周期调节的组素基因转录中的作用.
- 为了比较正常细胞与转化细胞中的HiNF-D活性.
- 阐明改变的HiNF-D活性与瘤转变之间的潜在联系.
主要方法:
- 从不同细胞周期阶段的细胞中提取的核蛋白质提取物的分析.
- 测试HiNF-D对H4基因素基因促进体的结合活性.
- 在正常双胞胎细胞中的HiNF-D活性与瘤衍生或转化细胞系的比较.
主要成果:
- 正常细胞表现出细胞周期依赖的HiNF-D结合活性,在S阶段达到峰值.
- 转化细胞在整个细胞周期中表现出构成性高的HiNF-D结合活性.
- 转化细胞中的HiNF-D活性仅在分化后降低.
结论:
- 改变,构成性HiNF-D与H4基因素基因促进体结合,与癌症中细胞生长调节的丧失相关.
- 这种HiNF-D的失调可能是导致瘤转变的关键机制.
- 准HiNF-D活动可能为癌症治疗提供治疗策略.
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