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Folding and Characterization of a Bio-responsive Robot from DNA Origami
Published on: December 3, 2015
在恒定温度下,DNA迅速折叠成纳米尺度的形状
Jean-Philippe J Sobczak1, Thomas G Martin, Thomas Gerling
1Physics Department, Walter Schottky Institute, Technische Universität München, Am Coulombwall 4a, 85748 Garching near Munich, Germany.
Science (New York, N.Y.)
|December 15, 2012
概括
数百个DNA链在恒定温度下迅速折叠成复杂的纳米级物体. 这种脱离平衡的DNA折叠过程实现了高产量,并与蛋白质折叠有相似之处.
科学领域:
- 生物物理学的生物物理.
- 纳米技术纳米技术
- 分子生物学分子生物学
背景情况:
- 自组装DNA是创建纳米结构的强大工具.
- 控制DNA折叠的动力学和热力学对于可预测的结果至关重要.
研究的目的:
- 为了研究DNA链在恒定温度下折叠的动力学.
- 探索序列,长度和拓对DNA折叠路径的影响.
- 为了实现复杂的3DDNA纳米结构的快速和高产量生产.
主要方法:
- 模板DNA链的合作折叠使用数百个较短的DNA链.
- 在不同温度下分析折叠和展开路径.
- 优化折叠条件 (温度,序列,丝长,拓).
主要成果:
- 数百个DNA链合作折叠长模板DNA成复杂的纳米物体在几分钟内在恒定温度下.
- 折叠发生在通过核化驱动的途径,受序列,长度和拓学的影响而脱离平衡.
- 展开发生在明显平衡的温度高于折叠.
- 优化的恒温导致快速生产3D DNA物体,产量接近100%.
结论:
- 恒温折叠使得快速,高产率的DNA自组装成复杂的纳米结构.
- 折叠过程与蛋白质折叠有相似之处,尽管有化学和结构上的差异.
- 这种方法促进了DNA纳米技术的实际应用.
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