相关实验视频
Updated: May 15, 2026

11:48
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Published on: October 9, 2014
脊椎动物物种中替代拼接的进化格局
Nuno L Barbosa-Morais1, Manuel Irimia, Qun Pan
1Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Ontario, Canada.
概括
种类的表型差异来自于替代拼接复杂性,而不仅仅是基因. 拼接模式迅速分离,甚至在类似的器官之间,突出其在脊椎动物进化中的作用.
科学领域:
- 进化生物学是进化的生物学.
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 尽管物种具有相似的蛋白质编码基因,但它们表现出显著的表型差异.
- 了解驱动这些表型差异的机制在进化研究中至关重要.
研究的目的:
- 研究替代拼接复杂性在脊椎动物物种之间表型多样化中的作用.
- 在进化时间内分析器官组织中的转录组差异.
主要方法:
- 来自各种脊椎动物物种的器官转录组的比较分析.
- 评估跨进化谱系的替代拼接复杂性和模式.
- 确定cis和trans作用的拼接调节机制.
主要成果:
- 在脊椎动物谱系中观察到替代拼接复杂性的显著变化,灵长类动物表现出最高的复杂性.
- 器官转录组迅速分离 (在600万年内),以反映物种身份,而不是器官类型.
- 大多数物种特异性拼接模式是cis导向的,但发现了一些跨作用的调节变化.
结论:
- 替代拼接复杂性是脊椎动物物种之间表型差异的关键驱动因素.
- 拼接形状的快速分歧对物种特异性特征作出了重大贡献.
- 拼接调节,包括跨作用因素,在进化多样化中起着至关重要的作用.
相关概念视频
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Pre-mRNA Processing: RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
