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Tagging and Fusion Proteins

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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Updated: May 15, 2026

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Published on: January 27, 2014

简化表达的蛋白质结合使用分裂的整因.

Miquel Vila-Perelló¹, Zhihua Liu, Neel H Shah

¹Department of Chemistry, Princeton University, Frick Laboratory, Princeton, New Jersey 08544, United States.

Journal of the American Chemical Society

|December 26, 2012

概括

此摘要是机器生成的。

这项研究引入了一种新的方法,使用工程分裂DnaE整因来有效制备蛋白质α-thioesters. 这一进步简化了用于研究和治疗应用的改性蛋白质的生成.

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相关实验视频

Last Updated: May 15, 2026

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Published on: January 27, 2014

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

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Published on: March 25, 2020

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

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Published on: April 26, 2017

科学领域:

生物化学生物化学
分子生物学分子生物学
蛋白质工程是指蛋白质工程.

背景情况:

化学修饰的蛋白质对于生物研究和治疗至关重要.
表达蛋白质结合 (EPL) 是一种常见的蛋白质修饰方法,但需要具有挑战性的蛋白质α-thioester生成.
目前用于重组蛋白α-thioester合成的现有方法通常是复杂和低效的.

研究的目的:

开发一种精简和高效的方法来制备重组蛋白α-thioesters.
为了利用工程分裂DnaE整因来改进蛋白质修饰策略.
为了证明这种新方法在蛋白质半合成中的应用.

主要方法:

用于蛋白质α-thioester制备的工程自然分裂的DnaE整因被使用.
开发了一种利用整体碎片亲和力的净化策略.
该方法应用于改性蛋白质的半合成,包括乙化素和单克隆抗体.

主要成果:

建立了一个新的,精简的程序,用于从细胞溶解物中产生和净化蛋白质α-thioesters.
与现有 inteins 相比,工程分裂的 DnaE inteins 显示出更快的动力学.
实现了复杂蛋白质的成功半合成,如修饰的抗体和组织蛋白.

结论:

开发的方法为生产蛋白质α-thioesters提供了一个有效的替代方案.
这种技术简化了蛋白质的修改,并扩大了其在生物研究和药物开发中的实用性.
工程分裂DnaE整因代表了推进蛋白质工程和治疗应用的强大工具.